GSM5573400: LN229 CTCF CrispR editing eCTCF rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Neural
Cell type
LN-229
Primary Tissue
Brain
Tissue Diagnosis
Glioma
Attributes by original data submitter
Sample
source_name
tumorigenic adherent glioma cell line (LN229)
cell type
tumorigenic adherent glioma cell line (LN229)
chip antibody
CTCF (#3418, Cell Signaling)
treatment
Treated with CrispR Cas9 LINC01116 (enhancer) sgRNA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7546S, NEB). ChIP-Seq with Drosophila spike-in chromatin (Cat. 53083, Active Motif) and Drosophila -specific histone variant, H2Av (Cat. 61686, Active Motif) were added to each sample prior IP for normalization.